Peptide reference
TB-500: Reconstitution Math & Reference
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How do you reconstitute TB-500?
TB-500 reconstitution math is identical to other lyophilized peptides: divide vial mass (mg) by added bacteriostatic water (ml) to get mg/ml. A 5 mg vial with 2 ml BAC water yields 2.5 mg/ml. Drawing 20 units on a U-100 syringe (0.2 ml) then delivers 500 mcg. Peptly does the conversion automatically.
What is TB-500?
TB-500 is a 17-amino-acid synthetic peptide fragment of Thymosin Beta-4, a regulatory peptide naturally present in most mammalian cells.
TB-500 has been studied in animal models exploring cell migration, angiogenesis, and tissue regeneration. It is not FDA-approved for human use.
TB-500 commonly ships in 2 mg, 5 mg, or 10 mg lyophilized vials. Reconstitution math follows the same vial-mass-over-water-volume formula as every other peptide.
In Peptly, switching to the TB-500 profile loads its compound notes and lets users save reconstitution mixes (e.g. "5 mg vial + 2 ml BAC = 2.5 mg/ml") for one-tap reload.
Reconstitution math — reference table
| Vial | BAC water | Concentration | Example dose | U-100 units |
|---|---|---|---|---|
| 2 mg | 1 ml | 2 mg/ml | 500 mcg | 25 units |
| 5 mg | 2 ml | 2.5 mg/ml | 500 mcg | 20 units |
| 5 mg | 3 ml | 1.67 mg/ml | 500 mcg | 30 units |
| 10 mg | 3 ml | 3.33 mg/ml | 1 mg | 30 units |
| 10 mg | 5 ml | 2 mg/ml | 1 mg | 50 units |
Storage and shelf-life
Most research protocols refrigerate reconstituted TB-500 at 2–8 °C and use within 2–4 weeks. Lyophilized powder typically stores at −20 °C.
How Peptly handles TB-500
- Pre-loaded compound profile — no manual entry of vial mass each time.
- Saved reconstitution mixes — reload any combination in one tap.
- Visual U-100 syringe view — see the draw mark before you pull it.
- Injection log — every shot, site, and cycle stored on-device.
- Smart reminders — schedule-aware notifications with site rotation prompts.
Other peptide references
- BPC-157 reconstitution math — BPC-157 is a synthetic pentadecapeptide — a 15-amino-acid fragment derived from a protective protein originally isolated from human gastric juice.
- Semaglutide reconstitution math — Semaglutide is a 31-amino-acid GLP-1 receptor agonist.
- Tirzepatide reconstitution math — Tirzepatide is a dual GIP/GLP-1 receptor agonist — a 39-amino-acid synthetic peptide.
- Ipamorelin reconstitution math — Ipamorelin is a 5-amino-acid synthetic peptide and a selective growth hormone secretagogue.
Related tools and guides
- Main peptide calculator overview
- Peptide reconstitution math reference
- BAC water calculator
- Step-by-step reconstitution guide
- Peptide dosage unit reference
- Peptide tracker — injection log
Frequently asked questions
How is TB-500 reconstitution different from Thymosin Beta-4? +
TB-500 is a 17-amino-acid fragment of Thymosin Beta-4 (which is 43 amino acids long). Reconstitution arithmetic is identical — both are dissolved in bacteriostatic water based on vial mass.
What concentration do most TB-500 research protocols use? +
Research protocols commonly target 2–5 mg/ml so that typical mass doses (500 mcg to 2 mg) draw between 10–50 units on a U-100 syringe — readable on a standard insulin scale.
Can Peptly track TB-500 cycles separately from BPC-157? +
Yes. The injection log records each shot against the peptide profile + saved reconstitution mix you chose at the time. You can see BPC-157 and TB-500 cycles side by side in the History view.
Why does the unit count change when I switch BAC water from 2 ml to 3 ml? +
More water dilutes the same vial mass to a lower mg/ml concentration. Lower concentration means more volume (more units) is needed for the same mass dose. Peptly recomputes this live as you adjust the BAC slider.
Is TB-500 approved for human use? +
No. TB-500 is not FDA-approved for human use. Peptly is a calculator and reference tool, not medical advice.